Data Science Fellow — Organoid Biochemistry

The role

The Postdoctoral Fellow will establish laboratory capacity for protein-focused biological support. This involves implementing APB-Display and membrane-active-polymer workflows to connect organoid phenotype and optical measurements to molecular mechanisms.

What you will do

• Establish routine molecular-biology, protein-expression, purification, conjugation, and biochemical-assay support for organoid projects.
• Implement APB-Display using various technologies including dual-functional hydrogel beads and sequencing-based readout.
• Develop focused nanobody and affinity-reagent libraries.
• Implement APB-SortSeq and APB-TiteSeq for quantitative screening.
• Validate selected binders and interaction measurements with orthogonal methods.
• Establish SMA/SMALP and related membrane-active-polymer screening.
• Apply quality-control methods for native nanodiscs.
• Coordinate targeted extraction and affinity enrichment of membrane proteins and complexes.
• Develop nanobody-enabled labeling, concentration, purification, and perturbation workflows.
• Create version-controlled SOPs, reagent inventories, and training modules.

Required qualifications

• Doctoral degree (PhD) in biochemistry, molecular biology, chemical biology, protein chemistry, biophysics, bioengineering, cell biology, or a closely related field.
• Demonstrated hands-on experience in recombinant protein expression and purification, molecular cloning, quantitative binding assays, or protein-interaction analysis.
• Strong understanding of protein structure-function relationships, membrane biology, assay controls, and quantitative experimental design.
• Ability to work with mammalian cells or complex biological samples.
• Ability to collaborate with imaging, spectroscopy, toxicology, computational, and core-facility teams.
• Ability to document methods rigorously.
• Ability to analyze data using Python, R, MATLAB, or comparable tools.
• Ability to communicate results through reports, presentations, and manuscripts.

Nice to have

• Experience with nanobodies, antibody engineering, display technologies, pooled libraries, FACS, next-generation sequencing, cell-free protein synthesis, microfluidics, or hydrogel beads.
• Experience with styrene-maleic-acid polymers, SMALPs, native nanodiscs, membrane-protein purification, lipidomics, quantitative proteomics, mass spectrometry, or electron microscopy sample preparation
• Experience with organoids, stem-cell-derived models, toxicology, chemical exposures, fluorescence imaging, or multi-omics integration.
• A strong record of method development, troubleshooting, collaborative publications, and independent experimental execution.

Benefits

• 100% Medical, Dental & Vision Coverage for Employees
• Paid Time Off and Paid Holidays
• 401K match up to 5%
• Educational Benefits for Career Growth
• Employee Referral Bonus
• Flexible Spending Accounts: Healthcare (FSA), Parking Reimbursement Account (PRK), Dependent Care Assistant Program (DCAP), Transportation Reimbursement Account (TRN)

About the company

Axle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications. They work with research organizations including multiple institutes at the National Institutes of Health (NIH).