Scientist, Cellular Immunology and Inflammation

The role

You will design, run and analyze cellular immunology and inflammation experiments that support target validation and mechanism-of-action studies for therapeutic discovery programs. The role is hands-on lab work in a team-based, matrixed environment at Prologue Medicines.

What you will do

• Design, run and analyze in vitro and ex vivo cellular immunology and inflammation experiments.
• Develop, optimize and implement cell-based assays for immune cell activation, signaling and inflammatory responses.
• Apply multiparameter flow cytometry, including panel design, staining, acquisition, analysis and cell sorting.
• Isolate, culture and manipulate primary human and/or animal immune cells using MACS and FACS.
• Design phenotypic and mechanism-of-action studies to validate therapeutic targets.
• Apply transcriptomic profiling and biomarker and cytokine assays to assess therapeutic activity.
• Analyze data, troubleshoot assay performance and recommend follow-up experiments.
• Collaborate with cross-functional research teams on discovery and translational strategies.
• Develop and maintain protocols, assay documentation and laboratory best practices.
• Present findings and recommendations to cross-functional teams and leadership.

Required qualifications

• PhD in Immunology, Cell Biology, Molecular Biology or a related biological discipline, or an M.Sc. with 6+ years of directly relevant experience in cellular immunology (or equivalent combination of ed
• Demonstrated hands-on expertise with patient-derived tissues and primary immune cells, including isolation, culture, stimulation, differentiation and functional characterization.
• Strong proficiency in multiparameter flow cytometry, including panel design, staining optimization, data acquisition and analysis.
• Proven experience developing, optimizing and troubleshooting cell-based functional and mechanistic assays.
• Strong understanding of immune cell biology and immune and inflammatory signaling pathways.
• Experience with singleplex and multiplex readouts such as MSD, Luminex, ELISA, ELISpot, AlphaLISA and TR-FRET.
• Ability to design experiments independently, critically evaluate results and translate findings into recommendations.
• Proficiency in quantitative data analysis and statistical interpretation, using tools such as FlowJo, GraphPad Prism, R or Python.
• Excellent organizational, problem-solving, communication and presentation skills.
• Ability to work independently and collaborate in team-based, matrixed, multidisciplinary research teams.

Nice to have

• Experience in biotechnology or pharmaceutical research supporting early-stage therapeutic discovery, target identification, validation and mechanism-of-action studies.
• Experience with bulk RNA-seq and, ideally, single-cell approaches (e.g., scRNA-seq, CITE-seq), plus qPCR and targeted expression panels.
• Familiarity with genomic analysis and bioinformatics, or the ability to partner with computational biologists.
• Experience with mammalian cell transfection and genetic perturbation (e.g., CRISPR, siRNA, lentiviral or electroporation in primary cells).
• Familiarity with 96- and 384-well plate formats, assay miniaturization and laboratory automation.
• Experience developing new experimental workflows or establishing assay platforms.
• Experience designing or contributing to in vivo immunology studies.
• Demonstrated scientific leadership, including mentoring junior scientists or research associates.

Benefits

• Healthcare coverage
• Annual incentive program
• Retirement benefits
• A broad range of other benefits (details not specified in the posting)

Before you apply

• Applications are reviewed using AI-assisted screening tools.
• Recruitment agencies may not submit unsolicited resumes; unsolicited agency resumes become the property of the company with no referral fee owed.

About the company

Prologue Medicines creates new medicines by unlocking the therapeutic potential of viral proteins, using its DELVE machine learning platform and the largest known database of viral protein structures. Prologue was founded by Flagship Pioneering in 2021.