Scientist / Senior Scientist, Library Prep

The role

You will lead research on the library preparation stage of a single-molecule protein sequencing platform. You will design and develop end-to-end library prep methods, troubleshoot mechanistic issues, and collaborate with multiple technical teams.

What you will do

• Own library prep method development for nanopore sequencing end to end
• Design and test new approaches at the interface between protein sequencing assay and library prep
• Develop on-bead preparation and clean-up methods for high-sensitivity sequencing
• Feed library prep requirements back into the design of upstream constructs and oligos
• Contribute to experimental design for ProSE studies and sequencing runs
• Work with automation to move manual workflows onto automated platforms
• Investigate and resolve yield-limiting artifacts from protein sequencing assay chemistry
• Use sequencing data to correlate library preparation variables with read counts and pore health
• Provide technical guidance and research experiment design to team members
• Work with oligo synthesis, assay development, integration, automation, and bioinformatics teams

Required qualifications

• PhD in Molecular Biology, Biochemistry, Bioengineering, Chemical Biology, or a related field, with 1+ years of industry experience, or an MS with 5+ years of relevant experience
• Experience developing or substantially re-engineering a sequencing library prep method for demanding templates
• Deep understanding of enzymatic DNA manipulation
• Experience working with chemically modified DNA or RNA
• Ability to reason from first principles to predict reagent and condition efficacy
• Proficiency with DNA characterization and QC methods such as capillary or gel electrophoresis
• Experience guiding the bench work of others
• Excellent problem-solving skills
• Strong attention to detail
• Excellent written and verbal communication skills
• Ability to thrive in a fast-paced environment with evolving priorities

Nice to have

• Hands-on experience with nanopore sequencing or another single-molecule DNA sequencing technology such as PacBio
• Experience characterizing the electrochemical properties of nanopores or related systems
• Experience with single-stranded DNA or RNA library prep, or PCR-free preparation with modified oligonucleotides
• Familiarity with automated liquid handling platforms (such as Hamilton, KingFisher)
• Background in protein sequencing, proteomics sample preparation, or peptide chemistry
• Comfort using AI tools (Claude, Claude Code)

Benefits

• Employee Stock Option Plan
• 100% Health Plan Coverage for Employees & Dependents (Medical, Dental, & Vision)
• Employer Retirement Contributions to 401(k)
• Generous Paid Time Off
• Paid Maternity and Paternity Leave
• Health & Wellbeing Program
• Office Snacks and Beverages
• Regular Team Bonding Activities

About the company

Glyphic Biotechnologies is developing a massively parallel, single-molecule proteome sequencing platform. They have raised >$80M from venture partners and non-dilutive grant funding.