Staff / Senior Scientist, Bioconjugation Chemistry

The role

You will design, develop, and execute bioconjugation strategies for peptide and oligonucleotide components. This work supports a single-molecule proteome sequencing platform.

What you will do

• Partner with sample prep and assay development to select conjugation chemistries
• Design bioconjugation workflows using NHS-ester, maleimides, isothiocyanate, and click chemistry
• Prepare bioconjugation reagents from proof-of-concept through prototype-kitting
• Develop labeling strategies based on Edman Chemistry
• Develop selective C-terminal and N-terminal conjugation chemistries
• Develop site-specific labeling strategies for amino acid sidechains
• Enable on-bead workflow to label and release peptides
• Synthesize and purify peptide and oligonucleotide conjugates
• Characterize conjugation efficiency, purity, and stability using HPLC, LC-MS/MS, NMR, CE, UV/Vis, and FTIR
• Scale conjugate synthesis from R&D quantities to prototype-grade supply

Required qualifications

• MS or PhD in organic chemistry, chemical biology, biochemistry, or a related field
• Industry experience in bioconjugation chemistry
• Ability to independently design, develop, and troubleshoot conjugation workflows
• 8+ years of experience for Staff Scientist (MS/PhD)
• 6+ years of experience for Senior Scientist (MS/PhD)
• Deep expertise in protein and/or peptide bioconjugation: NHS esters, maleimides, click chemistries (CuAAC, SPAAC, IEDDA), isothiocyanate coupling, and related bioorthogonal approaches
• Experience with site-specific amino acid sidechain chemistry beyond maleimide chemistry
• Track record developing novel blocking groups and conducting SAR studies
• Proficiency in analytical characterization: HPLC method development, LC-MS/MS, UV/Vis, and CE
• Method-development mindset

Nice to have

• Hands-on experience with solid-phase peptide synthesis (SPPS)
• Experience with peptide C-terminal chemistry manipulation methods
• Familiarity with oligonucleotide chemistry and oligo-conjugation techniques
• Experience with scalable surface chemistry methods for flow cells or solid-phase supports
• Familiarity with statistical and DOE-based experimental design
• Prior experience in an early-stage biotech or tools/diagnostics company

Benefits

• Employee Stock Option Plan
• 100% Health Plan Coverage for Employees & Dependents (Medical, Dental, & Vision)
• Employer Retirement Contributions to 401(k)
• Generous Paid Time Off
• Paid Maternity and Paternity Leave
• Health & Wellbeing Program
• Office Snacks and Beverages
• Regular Team Bonding Activities

About the company

Glyphic Biotechnologies is developing a massively parallel, single-molecule proteome sequencing platform. They have raised >$80M from venture partners and non-dilutive grant funding.