Staff / Senior Scientist, Assay Development (Chemistry)

The role

You will develop chemical methods for sample loading and build conjugation, cleavage, and stabilization workflows for a single-molecule proteome sequencing platform. You will work at the chemistry-assay interface to optimize bioconjugation and peptide labeling.

What you will do

• Optimize bioconjugation methods for peptide loading into the expansion assay
• Identify, characterize, and optimize peptide labeling during the ProSE sequencing assay
• Develop reagents and methods to maximize the stability of peptides, DNA, and peptide-DNA chimeras
• Partner with the Chemistry team to develop amino acid side-chain protection chemistries
• Evaluate compatibility of new chemistries with existing assay components
• Synthesize and test candidate reagents from literature and internal pipeline
• Design and determine the order of operations for chemical manipulations within the assay workflow
• Optimize selective cleavage chemistry workflows
• Develop stabilization strategies for sensitive assay intermediates and conjugates
• Employ analytical methods to characterize conjugation efficiency, adduct identity, purity, and stability

Required qualifications

• Advanced degree in organic chemistry, chemical biology, biochemistry, or a related field
• Industry experience designing multi-step chemical workflows in complex biological systems
• MS/PhD with 6+ years of experience for Senior Scientist
• MS/PhD with 8+ years of experience for Staff Scientist
• Demonstrated experience developing site-selective bioconjugation chemistry for native peptides
• Experience developing multi-step workflows using peptides, oligonucleotides and bioconjugation techniques
• Proficiency with analytical characterization of complex conjugates and assay intermediates: HPLC (RP and IEX), LC-MS/MS, UV-Vis
• Ability to function as both an independent contributor and a collaborative partner
• Excellent problem-solving skills and attention to detail
• Excellent written and verbal communication skills

Nice to have

• Familiarity with nanopore or single-molecule sequencing chemistry
• Experience with solid-phase peptide synthesis (SPPS)
• Familiarity with statistical and DOE-based experimental design for chemistry optimization
• Prior experience in an early-stage biotech or tools/diagnostics company

Benefits

• Employee Stock Option Plan
• 100% Health Plan Coverage for Employees & Dependents (Medical, Dental, & Vision)
• Employer Retirement Contributions to 401(k)
• Generous Paid Time Off
• Paid Maternity and Paternity Leave
• Health & Wellbeing Program
• Office Snacks and Beverages
• Regular Team Bonding Activities

About the company

Glyphic Biotechnologies is developing a massively parallel, single-molecule proteome sequencing platform to transform life science discovery. They have raised >$80M from venture partners and non-dilutive grant funding.