Co-op, Synthetic Biology (mRNA Therapeutics)

The role

This researcher will support the design, production, and in vitro testing of mRNA therapeutics. You will work with mammalian cell lines, transfect and test mRNAs, and support DNA template and mRNA production.

What you will do

• Maintain human and murine cell lines using sterile technique, passaging, cell counting and viability, and cryopreservation
• Transfect IVT mRNAs and LNP-formulated mRNAs into mammalian cells and quantify expression by flow cytometry
• Support DNA template design and construction including primer design, PCR, DNA assembly, plasmid prep and QC
• Support internal mRNA production and quality analysis including IVT, purification, Nanodrop/Bioanalyzer
• Execute, document in electronic lab notebook, and present experiments

Required qualifications

• B.S. or M.S. student in Biology, Biological Engineering, Molecular Biology, Biochemistry, or a related field
• Hands-on mammalian cell culture experience at the bench (sterile technique, passaging, transfection, viability assays)
• Able to work full-time (35-40 hours/week) on site in Boston's Seaport

Nice to have

• ≥6 months of previous laboratory experience
• Experience with molecular biology techniques (PCR, cloning, gel electrophoresis)
• Experience with assays such as flow cytometry

Benefits

• Free monthly subway pass
• Free Bluebikes membership
• Gym membership support
• On-site cafeteria

About the company

Kernal Bio is a venture-backed therapeutics company engineering cells inside the body using AI-designed, selective mRNA and targeted LNP technology. The company was founded by experts from MIT, Harvard, Merck, and BMS.